Table 2.
Real-time quantitative PCR primers for genes of L. vannamei.
| Gene | Primer sequence(5’ to 3’) | GenBank No. | Product size (bp) |
|---|---|---|---|
| β-actin F | CGAGGTATCCTCACCCTGA | AF300705.2 | 101 |
| β-actin R | CGGAGCTCGTTGTAGAAGG | AF300705.2 | |
| toll F | ATACCTCAGCTTCACG GCAG |
XM_027356519.1 | 140 |
| toll R | TATTCGTCAGCAGAGC AGGC |
XM_027356519.1 | |
| dorsal F | AGATGGAATGATAGAATG GGAAGC |
XM_027382195.1 | 127 |
| dorsal R | GTACACCTTTATGGGGTT CTCTATCTC |
XM_027382195.1 | |
| crustin F | GAGGGTCAAGCCTAC TGCTG |
AY486426.1 | 157 |
| crustin R | ACTTATCGAGGCCAG CACAC |
AY486426.1 | |
| sod F | GCCACTTGAACCACA CCATC |
DQ005531.1 | 158 |
| sod R | GCCAGAGCCTTTCAC TCCAA |
DQ005531.1 | |
| cat F | GGGTATTGAGGCTTCC CCTG |
AY518322.1 | 151 |
| cat R | GGGGCCATCTCTCTG GTAGT |
AY518322.1 | |
| gpx F | AGAAGAGTTCGGCGAC AAGC |
AY973252.2 | 126 |
| gpx R | TCGAAGTTGTTCCCA GGACG |
AY973252.2 | |
| imd F | TATACATCCTGCCGT TGCCG |
FJ592176.1 | 174 |
| imd R | GTTGTGGATAACGGGGC CAA |
FJ592176.1 | |
| relish F | ATTCTTCTGCGTTTCAAG GTGT |
KM204120.1 | 203 |
| relish R | GAGGTATGGTCAGGGTAT GGTG |
KM204120.1 | |
| lysc F | TACTGGTGCGGAAGC GACTA |
XM_027352840.1 | 165 |
| lysc R | GTAAGCCACCCAGGC AGAATA |
XM_027352840.1 |
β-actin, beta-actin; sod, superoxide dismutase; cat, catalase; gpx, glutathione peroxidase; imd, immune deficiency; lysc, lysozyme C-like.