Extended Data Fig. 9. A-to-G conversion efficiency of IscB- and SpG-based adenine base editors at endogenous loci in U-2OS and HeLa cells.
a, b, Comparisons of A-to-G conversion efficiency of IscB.m16*-ABE, enOgeuIscB-ABE, SpG-ABE at five target sites containing various TAMs in U-2OS (a) and HeLa (b) cells. Value and error bars are presented as means ± s.d., n = 3 independent biological replicates. c–f, Statistical of A-to-G editing efficiency (c, e) and indels (d, f) at five endogenous sites in U-2OS (c, d) and HeLa (e, f) cells, respectively. Value and error bars are presented as means ± s.d., n = 3 independent biological replicates. P values determined by Tukey’s multiple comparisons test following ordinary one-way analysis of variance. NS, not significant.