Extended Data Fig. 4. Characterization of IscB.m16 and IscB.m16* editing activities in HEK293T cells.
a, The indels patterns generated by wild-type IscB.m16 and its variants at VEGFA-S1 site. b, Cleavage activity of wild-type IscB.m16 and its variants are affected by spacer length at two target sites on GFxxFP reporters. c, Spacer length-dependent effects on cleavage activity in IscB.m16* and enOgeuIscB systems targeting two sites in human genome. Values and error bars were shown as mean and s.d., n = 3 independent biological replicates.