Extended Data Fig. 14. Transgenerational effects in the offspring of Rank^Δvil mothers.

a, Body weights of E18.5 male embryos of Rank^WT (n = 18) and Rank^ΔVil (n = 13) females fed normal chow. b, Body weights of male offspring born to Rank^WT (n = 20) and Rank^ΔVil (n = 23) dams fed normal chow. Mice were monitored from wk 1 to wk 24^th after birth. Each data point represents one offspring mouse. c, Body weights of 3 wks old Rank^WT and Rank^HET offspring born to Rank^WT and Rank^ΔVil dams. All pregnancies were syngeneic crosses with C57BL/6J males, thus the offspring of Rank^ΔVil dams were either Rank^WT (Rank^flox/WTVil^cre−) (n = 8) or Rank^Het (Rank^flox/WTVil^cre+) (n = 6); both of these groups exhibit reduced body weights as compared to Rank^WT(Rank^flox/WTVil^cre−) offspring (n = 17) born to Rank^WT dams. Dots represent individual mice. d, Numbers of offspring from Rank^WT (n = 48) and Rank^ΔVil (n = 54) dams. e, Body weights of male offspring born to Rank^WT (n = 18) and Rank^ΔVil (n = 16) dams fed high fat diet (HFD). f,g, Oral glucose-tolerance test (f) and corresponding area under the curve (AUC) (g) in male (n = 10/8) offspring of Rank^WT and Rank^ΔVil dams, fed normal chow for 25 wks. Box-and-whisker plots in g show the median (middle lines), interquartile range (boxes), and minimum to maximum values (whiskers) throughout. Data are mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant. Two-tailed Student’s t-test (a,d,g); Two-way ANOVA with Sidak’s multiple comparison (b); One-way analysis of variance (ANOVA) with Tukey’s post hoc test (c).

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