Table 1.

Primer sequences for validation and evaluation of amplification efficiency in RT-qPCR.

Gene1	Sequence (5′-3′)2	Product size, bp	Accession no.3	Tissues analyzed	Source
LAP	F: CCTGTCTGCTGGGTCAGGATTTA	134	NM_203435.4	Maternal small intestine	NCBI Primer-BLAST
	R: TTACTTGGGCTCCGAGACAGG
	F: GCCAGCATGAGGCTCCATC	194		Maternal mammary gland	(85–87)
	R: CTCCTGCAGCATTTTACTTGGGCT
EBD	F: TATAAAGCGGCAAGAGCAGCC	102	NM_175703.3	Maternal small intestine	(88, 89)
	R: AGCATTTTACTGAGGGCGTGA
BNBD4	F: CACAGCCTGCACAGAATTCCTC	172	NM_174775.1	Fetal and maternal lung	NCBI Primer-BLAST
	R: ACTCTTTGAGTAAATCCTGACCCA
BNBD5	F: CCTAGTCCTGTCTGCTGGGTC	122	NM_001130761.1	Fetal and maternal mammary gland	NCBI Primer-BLAST
	R: AGGTGCCAATCTGTCTCATGTTG
CATHL5	F: ACCTCCCAAGGAGGACGATG	152	NM_174510.3	Fetal mammary gland	NCBI Primer-BLAST
	R: TGACTGTCCCCACACACTCT
	F: TCGGGAGTAACTTCGACATCACCT	141		Fetal and maternal lung	(89, 90)
	R: GGCCCACAATTCACCCAATTCTGA
GAPDH	F: CTGCCCGTTCGACAGATAGC	153	NM_001034034.2	All	NCBI Primer-BLAST
	R: GATGGCGACGATGTCCACTT
ACTB	F: CCGCAAATGCTTCTAGGCGG	189	NM_173979.3	All	NCBI Primer-BLAST
	R: ACTGCTGTCACCTTCACCGT
HPRT1	F: CAGTTGCTGCATTCCCGAAC	125	NM_001034035.2	All	NCBI Primer-BLAST
	R: TTCCAGTCAATAGTGGTGTGGT

^{1LAP = lingual antimicrobial peptide, EBD = enteric β-defensin, BNBD4 = bovine neutrophil β-defensin4, BNBD5 = bovine neutrophil β-defensin5, CATHL5 = cathelicidin5. GAPDH = Glyceraldehyde-3-phosphate dehydrogenase. ACTB = Actin beta. HPRT1 = Hypoxanthine-guanine phosphoribosyltransferase1. 2F = Forward; R = Reverse. 3Accession numbers from NCBI database http://www.ncbi.nlm.nih.gov.}