Table 1.

Summary of demographic information and Mycobacterium bovis test results of six free-ranging vervet monkeys (Chlorocebus pygerythrus) sampled in the Greater Kruger Conservation Area, South Africa.

Case	Age	Sex	Sample date	Clinical signs	Macroscopic lesions present	Histological lesions present	Ziehl-Neelsen stain (cytology)	GXU®#	Speciation
1	Sub-adult	Female	28 Jun 2023	Depressed, cough, emaciated, weakness, incoordination	S, P, M, L	S, P, K	++	MTBC detected – medium; no RIF resistance	M. bovis
2	Adult	Female	1 Aug 2023	Depressed, emaciated, weakness	S, P, H, M	NA	+++	MTBC detected – high; no RIF resistance	M. bovis
3	Adult	Female	1 Feb 2024	Depressed, emaciated, weakness, visibly swollen lymph nodes	S, P, M, A	S (only tissue examined)	++	MTBC detected – high; no RIF resistance	M. bovis
4	Juvenile	Male	30 Mar 2024	Depressed, emaciated, weakness, visibly swollen lymph nodes, incoordination, dyspnoea	S, P, H, M, A, L	S, P, H, E	+++	MTBC detected – high; no RIF resistance	M. bovis
5	Adult	Female	30 Apr 2024	Depressed, emaciated, weakness, dyspnoea incoordination	S, P, M	S, P, K	+	MTBC detected – high; no RIF resistance	M. bovis
6	Sub-adult	Male	4 May 2024	Depressed, emaciated, weakness, dyspnoea	S, P, H, M, K	S, P, L, M, K	+	MTBC detected – medium; no RIF resistance	M. bovis

S, Spleen; P, Lungs & pulmonary lymph nodes (lnn), H, Head lnn; M, Mesenteric lnn; A, Mammary/axillary lnn; L, Liver; K, Kidney; E, enteritis; NA, not available; GXU, Cepheid Xpert MTB/RIF Ultra qPCR assay; RIF, rifampicin; *histopathologic analyses performed; #the levels of MTBC detected (very low/low/medium/high) by the Cepheid Xpert MTB/RIF Ultra qPCR assay were based on preprogrammed rpoB cycle threshold (Ct) values; very low (Ct > 28), low (Ct 22–28), medium (Ct 16–22) or high (Ct < 16).

+ 0–50 AFB per field; ++ 50–100 AFB per field; +++ > 100 AFB per field.