FIGURE 1.

Three-step RNAi screening of SNAREs involved in polarized transport in Drosophila photoreceptors. (A) Schematic diagram of RNAi screening of SNAREs involved in polarized transport. (B) Schematic diagram of the Gal4 expression pattern in developing eyes by eyeless–CoinFLP–Act5C–Gal4 and eyeless–CoinFLP–longGMR–Gal4. Alteration of the original schematic diagram in the study by Bosch et al. (2015). (C) Immunostaining of Syx5RNAi^JF03330-, Rab11RNAi^GD11761-, or Rab10RNAi^JF02058-expressing retina by eyeless–CoinFLP–Act5C–Gal4 and eyeless–CoinFLP–longGMR–Gal4 using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. GFP or RFP (red) and asterisks represent the cells expressing RNAi constructs. Scale bar: 5 μm (C).