FIGURE 5.

SNARE RNAi mosaic retinas showing category-III phenotypes (A, B). Immunostaining of Syx7RNAi^KK101990 (A) or Syx7RNAi^GD2767 (B)-expressing retina by eyeless–CoinFLP–longGMR–Gal4 using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. RFP (red) and asterisks represent the cells expressing RNAi constructs. (C, D) Immunostaining of Syx7 ¹ (C) or Syx7 ⁴ (D) mosaic retina using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. RFP (red) represents wild-type cells. Arrows indicate Rh1-containing large vesicles. (E–H) Immunostaining of SNARE RNAi construct-expressing retina by eyeless–CoinFLP–Act5C–Gal4 (E, F) or eyeless–CoinFLP–longGMR–Gal4 (G, H) using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. GFP or RFP (red) and asterisks represent the cells expressing RNAi constructs. (I, J) Immunostaining of Syx13 ^Δ11 (I) or Syx6 ^Δ5 (J) mosaic retina using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. RFP (red) represents the wild-type cells. (K–P) Immunostaining of SNARE RNAi construct-expressing retina by eyeless-CoinFLP–Act5C–Gal4 (M, O) or eyeless–CoinFLP–longGMR–Gal4 (K, L, N, P) using anti-Na⁺/K⁺-ATPase-α (green) and anti-Rh1 (blue) antibodies. GFP or RFP (red) and asterisks represent the cells expressing RNAi constructs. (K) SybRNAi^KK113351, (L) Bet1RNAi^HMJ22351, (M) Sec22RNAi^HMS01238, (N) Syx18RNAi^KK101345, (O) Use1RNAi^GLC01442, and (P) Sec20RNAi^HMS01172. Scale bar: 5 μm (A, B), 10 μm (C, D), 5 μm (E–H), 10 μm (I, J), and 5 μm (K–P).