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. 2024 Dec 23;15(12):927. doi: 10.1038/s41419-024-07313-1

Fig. 5. Arginine methylation of OGT at R348 promotes glycolysis in NSCLC.

Fig. 5

A H1299 cells transfected with CARM1 shRNAs or control were assessed by western blotting. O-GlcNAcylation levels in cells were assessed by western blotting. B H1299 cells were treated with increasing amounts of TP064 for 24 h. O-GlcNAcylation levels in cells were assessed by western blotting. C H1299 cells were transfected with Flag-OGT WT /R348K/R348F after knocking down endogenous OGT. O-GlcNAcylation levels of H1299 cells were assessed by western blotting. D The O-GlcNAcylation level was measured in H1299 cells re-expressing OGT WT/R348K after knockdown of endogenous OGT and treatment with TP064 for 24 h. O-GlcNAcylation levels in cells were assessed by western blotting. E, F ECAR (E) and lactic acid production (F) were measured in H1299 cells re-expressing OGT WT/R348K/R348F after knockdown of endogenous OGT (error bars indicate the mean ± SD, n = 3 experimental replicates, *P < 0.05, **P < 0.01, Student’s t test). G, H ECAR (G) and lactic acid production (H) were measured in H1299 cells re-expressing OGT WT/R348K after knockdown of endogenous OGT and treatment with TP064 for 24 h (error bars indicate the mean ± SD, n = 3 experimental replicates, ns = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test).