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. 2024 Nov 15;35(4):102390. doi: 10.1016/j.omtn.2024.102390

Figure 2.

Figure 2

Evaluation of VP64 and SAM systems for Gata4 activation in MEFs in MEFs and fCFs

(A) Schematic of the different systems tested. (B) Comparative analysis of the VP64 and SAM system for Gata4 activation in MEFs. (C) Schematic showing the location of the efficient sgRNA-targeted sites for Gata4 with all four efficient Gata4-sgRNAs (G-sgRNAs) located in −600 to 0 kb of the TSS for the Gata4 gene. (D and E) Transcriptional activation of endogenous Gata4 (Gata4_UTR) in MEFs (D) and fCFs (E) using the SAM system. (F and G) Western blotting analysis of GATA4 protein levels in MEFs with targeted activation (SAM-Gata4) or ectopic overexpression (pMx-Gata4) of Gata4 at various time points post-infection. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.