Figure 4.

Transcriptional activation of Tbx5 in MEFs and fCFs using the SAM system

(A) Schematic of sgRNA-targeted sites for Tbx5 with all three efficient Tbx5-sgRNAs (T-sgRNAs) located in −600 to 0 kb of the TSS for the Tbx5 gene. (B and C) Transcriptional activation of endogenous Tbx5 (Tbx5_UTR) in MEFs (B) and fCFs (C) using the SAM system. (D and E) Western blotting analysis of TBX5 protein expression in MEFs following targeted activation (SAM-Tbx5) or ectopic overexpression (pMx-Tbx5) at various time points post-infection. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.