Fig. 1.

APOE-ε4 BMEC had reduced barrier function. (a) Sequencing electropherograms of iPSC edited with CRISPR/Cas9 targeting the APOE gene. Red arrows indicate the nucleotide that determines the APOE genotype in APOE-ε3 isogenic control iPSC and edited APOE-ε4 iPSC. (b) TEER measured on APOE-ε3 and -ε4 hiBMEC (n = 18 samples per genotype). (c) Immunofluorescent labelling of tight junction proteins in APOE-ε3 versus APOE-ε4 hiBMEC. ZO-1 (red), occludin (magenta), and claudin-5 (green). (d) Percent continuous junctions quantified via Junction Analyser Program. Scale bar = 20 μm (n = 176–327 cells from 16 biological replicates). Dotted lines indicate interquartile range. Data analysed using Mann–Whitney tests.