Fig. 4.
Isotope labelling and13C metabolic flux analysis showed decreased glycolysis with redirection in the TCA cycle in APOE-ε4 hiBMEC. Fractional enrichment for M+2 isotopomers of (a) α-ketoglutarate (AKG), (b) fumarate, and (c) malate. Data pooled from two independent labelling experiments, n = 7. (d) Metabolic flux map showing ε4/ε3 flux ratios. Reversed fluxes are shown as dashed arrows. Fluxes without overlapping 95% confidence intervals shown with an asterisk under predicted flux ratio. Total ion counts for (e) pyruvate (f) AKG (g) palmitate and (h) GABA. All samples were normalised to mean of APOE-ε3 for the respective experiment. Data were pooled from two independent experiments (n = 7) and analysed with a Mann–Whitney test.