Fig. 6.

SIRT1 increased with post-training serum in APOE-ε3 hiBMEC and decreased with post-training serum in APOE-ε4 hiBMEC, but this did not change barrier integrity. (a, b) Representative Western blot and quantification of SIRT1 in hiBMEC treated with 20% genotype-matched serum for 24 h (n = 9 donors per condition). Representative confocal microscopy images of tight junction proteins (c) ZO-1 and (d) claudin-5 following serum treatment and (e, f) quantified using the junction analyser program (45 cells analysed per donor per treatment group, n = 9 donors per genotype). (g) TEER measurements in APOE-ε3 and -ε4 hiBMEC following 24 h of treatment with 20% genotype-matched serum. Data analysed using repeated measures two-way ANOVA with Fisher's Least Significant Difference Test.