Fig. 8.

Serum systemically altered the hiBMEC metabolome, but exercise training did not. (a) PLS-DA of the metabolite labelled fraction of APOE-ε3 (blue) and -ε4 (pink) hiBMEC treated for 24 h with 20% serum from genotype-matched individuals pre- (circle) and post- (triangle) exercise training and ¹³C₆-glucose. A line connects hiBMEC treated with serum samples from the same donor (n = 4 donors per genotype). (b) PLS-DA of the metabolite labelled fraction of APOE-ε3 (blue) and -ε4 (pink) hiBMEC treated for 24 h with no serum (circle) or serum (diamond). Serum effects were analysed for combined pre- and post-training serum samples and compared to hiBMEC not treated with serum (n = 8 donors per genotype with serum, n = 3 donors per genotype without serum).