Table 4.
Antibodies.
| Antibody | Manufacturer | Catalogue number | Dilution | RRID |
|---|---|---|---|---|
| GLUT1 | Thermo Fisher Scientific | 21829-1-AP | 1:100 (Immunostaining) 1:4000 (Western Blot) |
AB_10837075 |
| HK1 | Santa Cruz Biotechnology | sc-46695 | 1:1000 (Western Blot) | AB_627721 |
| HK2 | Santa Cruz Biotechnology | sc-374,091 | 1:1000 (Western Blot) | AB_10917915 |
| PFKFB3 | Cell Signaling Technology | 13123S | 1:1000 (Western Blot) | AB_2617178 |
| GAPDH | Thermo Fisher Scientific | 437,000 | 1:1000 (Western Blot) | AB_10374327 |
| LDH | Santa Cruz Biotechnology | sc-133,123 | 1:1000 (Western Blot) | AB_2134964 |
| β-Actin | Santa Cruz Biotechnology | sc-47778 | 1:1000 (Western Blot) | AB_626632 |
| INSR | Santa Cruz Biotechnology | sc-57342 | 1:1000 (Western Blot) | AB_784102 |
| SIRT1 | Cell Signaling Technology | 9475S | 1:1000 (Western Blot) | AB_2617130 |
| APOE | Thermo Fisher Scientific | MA516146 | 1:1000 (Western Blot) | AB_11157884 |
| GFAT | Cell Signaling Technology | 5322S | 1:1000 (Western Blot) | AB_10699031 |
| G6PD | Cell Signaling Technology | 12263S | 1:1000 (Western Blot) | AB_2797861 |
| Na/K ATPase | Santa Cruz Biotechnology | sc-28800 | 1:1000 (Western Blot) | AB_290063 |
| Akt | Cell Signaling Technology | 9272S | 1:1000 (Western Blot) | AB_329827 |
| p-Akt | Cell Signaling Technology | 9271S | 1:1000 (Western Blot) | AB_329825 |
| ZO-1 | Cell Signaling Technology | 13663S | 1:100 (Immunostaining) | AB_2798287 |
| Occludin | Cell Signaling Technology | 91131S | 1:100 (Immunostaining) | AB_2934013 |
| Claudin 5 | Thermo Fisher Scientific | 35–2500 | 1:100 (Immunostaining) | AB_2533200 |
| Anti-mouse IgG (H + L), HRP Conjugate | Promega | W4021 | 1:2000 (Western Blot) | AB_430834 |
| Anti-rabbit IgG (H + L), HRP Conjugate | Promega | W4011 | 1:2000 (Western Blot) | AB_430833 |
| Goat anti-rabbit IgG (H + L) 594 | Thermo Fisher Scientific | A-11012 | 1:1000 (Immunostaining) | AB_2534079 |
| Goat anti-mouse IgG (H + L) 488 | Thermo Fisher Scientific | A-11001 | 1:1000 (Immunostaining) | AB_2534069 |
| Hoechst | Thermo Fisher Scientific | PI62249 | 1:2000 (Immunostaining) | AB_2651133 |