Figure 2.

Deleting 116 amino acids from the C terminus leads to increased CTCF binding and affects gene expression

(A) CTCF and Rad21 ChIP-seq peaks at the cPCDH gene complex.

(B and C) Heatmaps of CTCF (B) and Rad21 (C) normalized signals at the cPCDH CBS elements. Student’s t test, ∗p < 0.05.

(D and E) Heatmaps of global CTCF (D) and Rad21 (E) ChIP-seq signals. Student’s t test, ∗∗∗∗p < 0.0001.

(F) QHR-4C interaction profiles of the PCDHα gene cluster using HS5-1 as an anchor.

(G) RNA-seq indicates increased expression levels of PCDHα6, PCDHα12, and PCDHαc2. Data are presented as mean ± SD; Student’s t test, ∗∗∗∗p < 0.0001.

See also Figures S1, S3, and S4.