Figure 1.

Bckdha deficient adipocytes maintain lipid droplet formation but display a metabolically compromised transcriptional profile. Characterization of sgControl and sgBckdha adipocytes 7 days post-induction of differentiation. A, Western blot of BCKDHA levels. B, brightfield images (scale bar = 400 μm). C, Mole percent enrichment of citrate from [U-¹³C₆]leucine (means ± SEM, n = 3, analysed via one-way ANOVA with Tukeys post hoc analysis, ∗∗∗p < 0.001 from post hoc analysis when compared to sgControl.). D, Volcano plots of differentially expressed genes in Bckdha-deficient adipocytes compared to Control adipocytes. E, gene set enrichment analysis of Bckdha-deficient adipocytes generated using WEB-based GEne SeT AnaLysis Toolkit (WebGestalt). A–C, results are depicted from one representative experiment which was repeated independently at least three times.