Figure 2.

Bckdha deficient adipocytes reprogram central carbon metabolism.A, molar amount of glucose uptake in Control or Bckdha-deficient adipocytes over 48 h. B, molar amount of lactate secretion in Control or Bckdha-deficient adipocytes over 48 h. C, heat map of the abundance of intracellular metabolites in Bckdha-deficient adipocytes, statistical analysis via one-way ANOVA (9 cellular replicates). FDR∗<0.05, ∗∗<0.01, ∗∗∗<0.001. D, mole percent enrichment (MPE) of TCA cycle intermediates after 48 h of incubation with [U-¹³C₆]glucose. E MPE of TCA cycle intermediates after 48 h of incubation with [U-¹³C₅]glutamine. F, normalized oxygen consumption rate of 3T3-L1 Control or Bckdha-deficient adipocytes treated with the indicated pharmacological inhibitors (n = 3 experiments internally normalized to sgControl). G, ATP-linked respiration (n = 3 individual experiments internally normalized to sgControl). A, B, D and E, data are presented as means ± SD with three cellular replicates. Results are depicted from one representative experiment which was repeated independently at least three times. (A, B, D–G) One-way ANOVA with Tukeys post hoc analysis for comparison of each groups means. Significance in all is from post hoc analysis and compared to sgControl. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.