Figure 4.

Bckdha deficiency alters lipogenic acetyl-CoA sourcing and fatty acid diversity.A, contribution of [U-¹³C₆]glucose to the lipogenic AcCoA pool used for de novo lipogenesis (DNL) as determined via ISA. B, relative fold change in total odd- and even-straight-chain fatty acids in Bckdha-deficient 3T3-L1 adipocytes normalized to Control adipocytes. C, relative fold change in total branched-chain fatty acids (BCFAs) in Bckdha-deficient 3T3-L1 adipocytes normalized to Control adipocytes. D, molar amount of indicated fatty acids synthesized over 48 h obtained by combining individual FA DNL values with pool size. E, desaturation index in Control and Bckdha-deficient 3T3-L1 adipocytes. Data are presented as means ± SD (B–D), means with 95% confidence interval (C.I.) (A), and means ± SEM (E) with three cellular replicates. Each parameter was analysed via one-way ANOVA with Tukeys post hoc analysis except A, where significance is denoted as non-overlapping 95% C.I. Significance in all is compared to sgControl. Results are depicted from one representative experiment which was repeated independently at least three times. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.