REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
MitoTracker	Invitrogen	M22426
Hoechst 33342 trihydrochloride	Invitrogen	H3570
Molecular Probes Wheat Germ Agglutinin Concanavalin A, Alexa Fluor® 488 Conjugate	Invitrogen	C11252
SYTO® 14 Green Fluorescent Nucleic Acid Stain	Invitrogen	S7576
Alexa Fluor® 568 Phalloidin	Invitrogen	A12380
Alexa Fluor 555 Conjugate	Invitrogen	W32464
Chemicals, peptides, and recombinant proteins
Y-39983 HCl	SelleckChem	S7935
Pemigatinib (INCB054828)	SelleckChem	S0088
CP21R7 (CP21)	SelleckChem	S7954
Paraformaldehyde	Electron Microscopy Sciences	15710-S
HBSS	Thermo Fisher Scientific	14025126
Triton X-100	Sigma-Aldrich	T8787
DMSO	Sigma	D2650
Phosphate Buffered Saline (PBS)	Gibco™	10010072
Propidium Iodide	ThermoFisher	P3566
TrypLE Select	ThermoFisher	12604013
Synthafreeze	ThermoFisher	A1254201
SNPTrace™ Assay	Standard BioTools	100–6280
mycoplasma kit	Lonza	LT07-703
Experimental models: Cell lines
Cell Lines	NYSCF Repository
Software and algorithms
ScaleFEx	https://github.com/NYSCF/ScaleFEx	https://doi.org/10.5281/zenodo.13928509
CellProfiler	https://cellprofiler.org/
Distributed CellProfiler	https://github.com/DistributedScience/Distributed-CellProfiler
Other
ScaleFEx validation data: raw images	https://nyscfopensource.blob.core.windows.net/nyscfopensource/scalefex/ScaleFExDataset.zip
ScaleFEx features from the validation data with associated metadata	https://nyscfopensource.blob.core.windows.net/nyscfopensource/scalefex/scalefex_raw_features.parquet.
ScaleFEx features normalized, corrected, uncorrelated and well-level averaged, with associated metadata	https://github.com/NYSCF/ScaleFEx/blob/main/demos/ScaleFEx_corrected_averaged_features.csv
RxRx2 dataset	https://www.rxrx.ai/rxrx2