Figure 3.

OGR1 is involved in the acid-induced increase of Ca²⁺ levels in osteoclasts (n=6). (A) Representative fluorescence change showing the elevation of Ca²⁺ levels in acidic medium at pH 6.8. (B) Chelation of extracellular calcium with EGTA (0.5 mM) did not inhibit acid-induced Ca²⁺ increase. (C) Restrain acid-induced elevation of Ca²⁺ levels with BAPTA-AM (50 mM). (D) Acid-induced elevation of Ca²⁺ levels in osteoclasts was reduced by the OGR1 inhibitor. (E) Inhibition of phospholipase C with U73122 (10 mM) suppressed the acid-induced rise of Ca²⁺.