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. 2024 Dec 3;29(2):27. doi: 10.3892/etm.2024.12777

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Copyright: © 2024 Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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EGFR-AS1 plays the role of ceRNA and regulates HDAC1 with miR-449a. (A) ENCORI predicted the EGFR-AS1 and the miR-449a binding sites of the HDAC1 3'-UTR the respective wild type and mutated luciferase reporter vectors. (B) Luciferase assay for EGFR-AS1 and miR-449a for the specific association of EGFR-AS1 with miR-449a. (C) Luciferase assay test for the specific association of miR-449a with HDAC1 3'-UTR. (D) Ago2-RIP enrichment for EGFR-AS1 and miR-449a. (E) SNRNP70-RIP (positive control). ce, competing endogenous; HDAC1, histone deacetylase 1; miR, microRNA; Ago, argonaute-; RIP, RNA immunoprecipitation.