Figure 1.

Experimental flow chart. A total of 51 Guizhou yellow chickens were collected. The intramuscular fat (IMF) content of breast muscle of all chickens was measured. Cecal contents and serum samples were collected for microbial 16S rRNA gene sequencing and non-targeted metabolome detection, respectively. High- and low-IMF chicken groups were established in the experimental cohorts. The differences in the cecal microbiota and metabolome between two groups were compared and the correlation analysis of differential microbes and differential metabolites was conducted.