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PCR target fragment size: WT (wild type): one band at 178 bp. Heterozygous (flox/ +): 2 bands at 252 bp and 178 bp. Homozygous (flox/flox): 1 band at 252 bp. Cre amplicon: 180 bp. a PCR results: NCSTN(flox/ +) mice successfully obtainhad that it had 2 bands at 252 bp and 178 bp. b PCR results: CAGGCre–ERTM mice was verified that it had 1 band at 180 bp
