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. 2024 Dec 4;22(2):25. doi: 10.3892/br.2024.1903

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Copyright: © 2024 Hattori and Shimizu.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of the charge ratios of TC-1-12-based mRNA lipoplexes on cytotoxicity in HeLa cells transfected with the FLuc mRNA lipoplexes. FLuc mRNA lipoplexes were prepared using the MEI and TFH methods at charge ratios of 1:1 to 6:1, transfected into HeLa cells, and incubated for 24 h. Lipofectamine MessengerMAX was used as a control. Each value is represented as the mean ± standard deviation (n=5 for 5:1 (MEI), 1:1 (TFH), and 3:1 (TFH), and n=6 for other groups). ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. TFH method. TC-1-12, 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino)-N,N,N-trimethyl-11-oxoundecan-1-aminium bromide; FLuc, firefly luciferase; MEI, modified ethanol injection; TFH, thin-film hydration.