Figure 6.

Effect of the storage of the lipid-ethanol solution on Luc expression in HeLa cells transfected with the TC-1-12-based FLuc mRNA lipoplexes. Lipid-ethanol solution was stored at -20, 4, and 37˚C for 4 months. FLuc mRNA lipoplexes were prepared at a charge ratio of 3:1 using the MEI method, transfected into HeLa cells, and incubated for 24 h. Lipofectamine MessengerMAX was used as a control. Each column represents the mean ± standard deviation (n=3). TC-1-12, 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino)-N,N,N-trimethyl-11-oxoundecan-1-aminium bromide; N.S., not significant; FLuc, firefly luciferase; MEI, modified ethanol injection.