Figure 8.

Protein expression and cytotoxicity in HepG2 cells transfected with the mRNA lipoplexes. (A and C) FLuc mRNA and (B) EGFP mRNA lipoplexes were prepared at a charge ratio of 3:1 using the MEI method, transfected into HepG2 cells, and incubated for 24 h. Lipofectamine MessengerMAX was used as a control. (A) Luc activity was measured 24 h after transfection. Each column represents the mean ± standard deviation (n=3). ^***P<0.001. (B) EGFP expression in cells was observed 24 h after transfection using a fluorescence microscope. Scale bar, 100 µm. (C) cell viability was measured 24 h after transfection. Each column represents the mean ± standard deviation (n=6 for Lipofectamine MessengerMAX; n=7 for other groups). ^*P<0.05 and ^***P<0.001. Luc, luciferase; FLuc, firefly luciferase; EGFP, enhanced green fluorescent protein; MEI, modified ethanol injection