Table I.
Size of TC-1-12-based mRNA lipoplexes prepared using MEI and TFH methods.
| Preparation method | Charge ratio | Sizea (nm) | PDI | ζ-potentiala (mV) |
|---|---|---|---|---|
| MEI | 1:1 | 139.1±2.1 | 0.18±0.02 | -24.5±1.0 |
| 2:1 | 237.5±3.9 | 0.11±0.01 | 5.2±0.1 | |
| 3:1 | 115.8±1.1 | 0.11±0.00 | 1.7±3.1 | |
| 4:1 | 80.8±0.7 | 0.20±0.02 | 6.1±0.6 | |
| 5:1 | 86.3±0.1 | 0.19±0.02 | 8.4±0.2 | |
| 6:1 | 80.0±1.1 | 0.17±0.01 | 9.9±0.9 | |
| TFH | Only liposome | 104.7±0.8 | 0.25±0.00 | 54.2±1.6 |
| 1:1 | 275.4±9.6 | 0.24±0.01 | 11.8±0.7 | |
| 2:1 | 186.9±3.7 | 0.24±0.01 | 18.5±1.2 | |
| 3:1 | 183.9±12.3 | 0.21±0.05 | 27.0±1.9 | |
| 4:1 | 165.3±29.1 | 0.18±0.08 | 44.1±3.5 | |
| 5:1 | 216.4±21.1 | 0.11±0.00 | 44.0±0.8 | |
| 6:1 | 198.2±23.1 | 0.13±0.04 | 47.1±1.3 |
aIn MEI method, PBS solution containing FLuc mRNA lipoplexes was diluted three times with water. In TFH method, FLuc mRNA lipoplexes suspended in water were diluted with an appropriate amount of water. Each value represents the mean ± standard deviation (n=3). PDI, polydispersity index; MEI, modified ethanol injection; TFH, thin-film hydration.