Table 1.
Loci | Primers and base pairs (5′ to 3′) | References |
---|---|---|
LSU (large subunit rDNA) | Forward: LROR 5′-GTACCCGCTGAACTTAAGC-3′ Reverse: LR5 5′-ATCCTGAGGGAAACTTC-3′ |
Vilgalys and Hester (1990) |
ITS (internal transcribed spacers) | Forward: ITS5 5′-TCCTCCGCTTATTGATATGC-3′ Reverse: ITS4 5′-GGAAGTAAAAGTCGTAACAAGG-3′ |
White et al. (1990) |
tef1-α (elongation factor 1-alpha) | Forward: EF1-728F 5′-CATCGAGAAGTTCGAGAAGG-3′ Reverse: EF-2 5′-GGARGTACCAGTSATCATGTT-3′ |
O'Donnell et al. (1998); Carbone and Kohn (1999) |
tub2 (β-tubulin) | Forward: Bt2a 5′-GGTAACCAAATCGGTGCTGCTTTC-3′ Reverse: Bt2b 5′-ACCCTCAGTGTAGTGACCCTTGGC-3′ |
Glass and Donaldson (1995) |