Skip to main content
. 2024 Dec 11;14:1476066. doi: 10.3389/fcimb.2024.1476066

Table 1.

LSU, ITS, tef1-α, and tub2 loci primers information.

Loci Primers and base pairs (5′ to 3′) References
LSU (large subunit rDNA) Forward: LROR 5′-GTACCCGCTGAACTTAAGC-3′
Reverse: LR5 5′-ATCCTGAGGGAAACTTC-3′
Vilgalys and Hester (1990)
ITS (internal transcribed spacers) Forward: ITS5 5′-TCCTCCGCTTATTGATATGC-3′
Reverse: ITS4 5′-GGAAGTAAAAGTCGTAACAAGG-3′
White et al. (1990)
tef1-α (elongation factor 1-alpha) Forward: EF1-728F 5′-CATCGAGAAGTTCGAGAAGG-3′
Reverse: EF-2 5′-GGARGTACCAGTSATCATGTT-3′
O'Donnell et al. (1998); Carbone and Kohn (1999)
tub2 (β-tubulin) Forward: Bt2a 5′-GGTAACCAAATCGGTGCTGCTTTC-3′
Reverse: Bt2b 5′-ACCCTCAGTGTAGTGACCCTTGGC-3′
Glass and Donaldson (1995)