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. 2024 Dec 24;15(12):929. doi: 10.1038/s41419-024-07316-y

Fig. 5. Reduced BAHD1 expression improved the radiosensitivity of 22Rv1-RR and FaDu-RR cells relative to the parental WT cells.

Fig. 5

A A hypothesised model showing BAHD1 contributes to the enhanced heterochromatin response in RR cancer cells, whereby increased repair efficiency following irradiation (IR)-induced damage leads to decreased radiosensitivity. The inhibition of BAHD1 leads to chromatin unpacking, decreased repair efficiency and improved radiosensitivity (created with Biorender.com). B and C, left Representative images of co-localised γH2AX (green) and p-53BP1 (red) foci in 22Rv1-RR and FaDu-RR cells at 1 and 6 h post-IR, with and without siBAHD1 treatment. Scale bar: 5 μm; (right) Quantification of co-localised γH2AX and p-53BP1 mean foci per cell, bars represent mean±SD, n = 3 per group. D, E Representative western blot showed the changes in the expression of DSB, DNA repair, cell cycle arrest and heterochromatin markers of 22Rv1-RR cells and FaDu-RR cells at 1 and 6 h post-IR, with and without siBAHD1 treatment (normalised against control).