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. 2024 Nov 18;45(6):1249–1260. doi: 10.24272/j.issn.2095-8137.2024.105

Figure 1.

Figure 1

Design of CRISPR-Cas13 constructs for RNA manipulation in the silkworm

A: Schematic of piggyBac-based plasmids for ectopic expression of PspCas13b and CasRx in silkworm BmE cells and living individuals. B: Schematic of piggyBac-based plasmids expressing crRNAs for targeting Cas13 variants to different genes. pBac, piggyBac; ITR, inverted terminal repeats of piggyBac; EGFP, enhanced green fluorescent protein; Puro, puromycin resistance selection marker; 3×P3, eye-specific artificial promoter; IE1, ubiquitous baculovirus immediate early 1 gene promoter; U6, polymerase III U6 promoter; SV40, SV40 poly(A) terminator; 2A, 2A peptide; NLS, nuclear localization signal; HA, HA tag.