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. 2024 Nov 18;45(6):1249–1260. doi: 10.24272/j.issn.2095-8137.2024.105

Figure 2.

Figure 2

Establishment of stable silkworm BmE cell lines expressing Cas13 variants

A–D’: Microscopy analysis of BmE cells expressing EGFP-fused Cas13 proteins. White light microscopy (A–D) and fluorescence microscopy (A’–D’) were conducted. Scale bar: 50 μm. E–H: Flow cytometry analysis of BmE cells expressing EGFP-fused Cas13 proteins. Control, original BmE cell without transfection. I, J: Western blotting validation of expressions of HA-tagged Cas13 proteins in BmE cells. Anti-HA and anti-Tubulin antibodies were used. EGFP, enhanced green fluorescent protein; Puro, puromycin selection marker gene; HA, HA tag.