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. 2024 Nov 18;45(6):1249–1260. doi: 10.24272/j.issn.2095-8137.2024.105

Figure 5.

Figure 5

CRISPR-CasRx-mediated Scr knockdown in silkworm larvae

A, B: CRISPR-CasRx-mediated editing of the Scr RNA resulted in a decrease in the size and weight of larval body. Scale bar: 1 cm. Values are mean±SE (error bars) from three independent biological replicates. Different letters indicate significant differences at P<0.05, determined by one-way ANOVA followed by Tukey test. C–J: Scr RNA editing decreased the size of larval wing disc (C, D, H), silk gland (E, I), fat body cells (F, G, J). Scale bar: 1 cm for silk gland; 50 μm for fat body cells; 200 μm for wing disc. K: Scr RNA editing had no effect on the duration of larval development. Gray areas denote larval molting. L, larval instars; L1 to L5 represent first to fifth larval instars. Values are mean±SE (error bars) from three independent biological replicates. Significant differences were determined using Student’s t-test, **: P<0.01 and ***: P<0.001 versus WT as the control. WT, wild-type.