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. 2024 Nov 18;45(6):1261–1275. doi: 10.24272/j.issn.2095-8137.2024.125

Figure 6.

Figure 6

LncRNA GTL2 inhibits proliferation of ovine skeletal muscle satellite cells (SCs)

A: Knockdown of lncRNA GTL2 utilizing RNA interference (RNAi). B: CCK-8 assay showing cell vitality of SCs transfected with negative control or si-lncRNA GTL2. C: EdU assay detecting proliferation of SCs after knockdown of lncRNA GTL2. D: Numbers of cells in Gap 1 phase (G1), synthesis phase (S), and Gap 2 phase (G2) were calculated by flow cytometry of si-lncRNA GTL2. E: Western blot assay of CDK1 and CDK2 in SCs transfected with si-lncRNA GTL2. F: Cell transfection efficiency of over-lncRNA GTL2. G: CCK-8 assay showing cell vitality of SCs transfected with empty-pcDNA3.1(+) or over-lncRNA GTL2. H: EdU assay detecting proliferation of SCs after overexpression of lncRNA GTL2. I: Numbers of cells in Gap 1 phase (G1), synthesis phase (S), and Gap 2 phase (G2) were calculated by flow cytometry of over-lncRNA GTL2. J: Western blot assay of CDK1 and CDK2 in SCs transfected with over-lncRNA GTL2. Results are presented as mean±SEM, *: P<0.05; **: P<0.01; ***: P<0.001.