Figure 7.

LncRNA GTL2 promotes differentiation of ovine skeletal muscle satellite cells (SCs)

A: Western blot assay of MyoG, MyoD, and SRF in SCs transfected with negative control or lncRNA GTL2 siRNA. B: Western blot assay of MyoG, MyoD, and SRF in SCs transfected with empty-pcDNA3.1(+) or over-lncRNA GTL2. C: Representative myotube staining of differentiated SCs transfected with si-lncRNA GTL2. Cells were stained with DAPI (blue) and MyHC (green) to visualize nuclei and myotubes, respectively. D: Immunofluorescence after transfection with over-lncRNA GTL2. SCs were differentiated for 3 days in differentiation medium. Results are presented as mean±SEM, ^*: P<0.05; ^**: P<0.01.