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. 2024 Dec 25;28(24):e70293. doi: 10.1111/jcmm.70293

FIGURE 6.

FIGURE 6

Smooth muscle cell switch from contractile phenotype to synthetic phenotype in AD aortas. (A) Immunostaining of CNN1 (contractile VSMC marker) and OPN (synthetic VSMC marker) in control and AD aortas. Mean fluorescence intensity of immunostaining was quantified. **p < 0.01, N = 3. (B) Western‐blotting detecting contractile VSMC markers (CNN1, α‐SMA) and synthetic VSMC marker (OPN) in AD and control aortas. ***p < 0.01, N = 3. (C) Immunostaining of CNN1 (contractile VSMC marker) and OPN (synthetic VSMC marker) in control‐VSCs‐VSMCs and AD‐VSCs‐VSMCs. Mean fluorescence intensity of immunostaining was quantified. **p < 0.01, N = 3. (D) Western‐blotting detecting protein expression levels of contractile VSMC markers (CNN1, α‐SMA) and synthetic VSMC marker (OPN) in control‐VSCs‐VSMCs and AD‐VSCs‐VSMCs. *p < 0.05, ***p < 0.01, N = 3.