Figure 5.

Morphometric analyses of GFAP positive astrocytes in the hippocampi of AAV transduced mice. (A) Representative GFAP images of hippocampal slices of mice injected with PBS, P301L-GFP AAV alone, or P301L-GFP AAV in combination with SUMO2-RFP AAV stained with GFAP (scale bar 500 μm), 40x magnification (middle panels, scale bar 50 μm) corresponding to the squares and 60x magnification highlighting 2–3 cells in the brain tissue (right panels, scale bar 20 μm). The quantification of the number of GFAP+ cells/mm2 (B), the percentage of GFAP stained area (C), and the astrocyte average size (D) reveal that Tau expression induces an atrophy of astrocytes that is prevented by SUMO2 co-transduction; one-way ANOVA followed by Tukey’s multiple comparisons test; * vs. CTR or # vs. P301L + SUMO2, # or *p < 0.05, ## or **p < 0.001, n = 4, 10 and 8.