Figure 1.

Exploration of the optimal concentration and time of H₂O₂ and baicalin. (A) Effect of different concentrations of H₂O₂ (0, 100, 200, 400, 600, 800, and 1,000 μM) on the cell viability of BMECs upon stimulation for 4, 6, and 8 h. *P < 0.05 compared with the CON, **P < 0.05 compared with the 200 μM H₂O₂ group, ^#P < 0.05 compared with the 400 μM H₂O₂ group. (B) Effect of different concentrations of H₂O₂ on ROS levels in BMECs upon stimulation for 6 h. *P < 0.05. (C) Effect of different concentrations of baicalin (0, 5, 10, 25, 50, 100 μM) on the cell viability of BMECs upon stimulation for 12, 24 and 48 h. *P < 0.05 compared with the CON, **P < 0.05 compared with the 10 μM baicalin group, ^#P < 0.05 compared with the 50 μM baicalin group. (D) BMECs were treated with baicalin at different concentrations, and 200 μM H₂O₂ was added 6 h before the end of treatment to determine the cell viability at 24 h. *P < 0.05. (E) Effect of different concentrations of baicalin on H₂O₂-induced ROS levels in BMECs. *P < 0.05. The data from the CON were used to normalize the data of each treatment group. Each treatment was repeated three times, and the results are expressed as the means ± SEM.