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. 2024 Dec 12;15:1511638. doi: 10.3389/fphys.2024.1511638

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Copyright © 2024 Somogyi, Tóth, Ballók, Hammad, Hussein, Kun-Szabó, Tolnai, Danis, Kecskés, Fodor, Farkas and Peták.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme of the experimental protocol. Rats in ventilated study groups randomly underwent bilateral occlusion of the common carotid artery (Group 2VO), unilateral occlusion of the middle cerebral artery (Group MCAO), or sham operation (Group SHAM); These animals were used to assess static lung volume (EELV), forced oscillatory respiratory mechanics (FOT), methacholine responsiveness (MCh), determination of brain infarct size (TTC), and histological changes in the brain. Non-ventilated animals received the same interventions; these rats were used to measure systemic inflammatory responses from blood serum, and organ-specific inflammation in lung tissue and bronchoalveolar lavage fluid (BALF) without the confounding effect of mechanical ventilation.