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. 2024 Dec 12;15:1511638. doi: 10.3389/fphys.2024.1511638

FIGURE 3.

FIGURE 3

Confirmation of ischemic injury caused by cerebrovascular occlusions. Panel (A) representative images of brain slices stained with triphenyl-tetrazolium chloride (TTC) demonstrating ischemic infarcts (unstained area). Scale bar = 1 cm. Panel (B) Estimation of relative infarct size. N = 5 for each group. Panel (C) schematic representation of the brain sections indicating the regions of interest for the quantification of immunolabeling. Panel (D) representative images of brain sections immunostained for MAP-2, GFAP, and Iba-1 from the frontal cortex and the striatum. Scale bar = 100 µm. Panel (E) quantification of the relative surface covered by the immunolabeling of MAP-2 and GFAP, and the ramification index of Iba-1 positive microglia of each region of the brain. N = 6 for each group. SHAM: sham surgery, 2VO: bilateral common carotid artery occlusion, MCAO: middle cerebral artery occlusion; MCAO CL: contralateral hemisphere to MCAO; MCAO IL: ipsilateral hemisphere to MCAO; FR, frontal cortex; STR, striatum; MAP-2, microtubule-associated protein 2; GFAP, glial fibrillar acidic protein; Iba-1, ionized calcium-binding adapter molecule 1. *: p < 0.05.