Figure 1.

Combined therapeutic efficacy of aloe-emodin (AE) and TIENAM (imipenem and cilastatin sodium for injection, TIE) in a mouse model of CLP-induced sepsis. (A) Schematic representation of the experimental protocol. (B) Illustration of typical behavioral alterations observed in mice following a 12-h regimen of AE (10 mg/kg/day) and TIE (50 mg/kg/day) administration. (C) Assessment of the murine sepsis score (MSS) in septic mice following 12 h of concurrent AE and TIE treatment (n = 10). Data are expressed as mean ± SEM. Statistical analysis was conducted using ANOVA followed by Tukey's post hoc test. **P < 0.01, ***P < 0.001, and ****P < 0.0001. (D) Impact of AE and TIE administration on the survival of the sham group (n = 10). (E) Survival outcomes of septic mice subsequent to the treatment (n = 20). Survival analysis was performed using the Mantel–Cox test; *P < 0.05 and ***P < 0.001; ns, not significant. Sample sizes are indicated within parentheses.