Total levels mTOR (A), Rheb (B) and LAT1 (C) in the cytosolic fraction and total levels of mTOR (D), Rag B (E) and LAMP2 (F) in the lysosomal fraction before and after resistance exercise and intake of EAA in Young and Old. White bars represent the resting leg in both groups, whereas grey and black bars represent the exercising leg in Young and Old, respectively. A three‐way (age × time x leg) repeated‐measures ANOVA was used to analyse the data. The ANOVA revealed a significant interaction effect (age × time × leg) for (A) and a main effect of age for (B,C). Furthermore, the ANOVA revealed a significant interaction effect (time × leg) for (D) and main effects of age as well as leg for (E). The symbols marked without lines represent a three‐way interaction, symbols marked with short lines represent a two‐way interaction, and symbols marked with a long line represent a main effect; *p < 0.05 different from baseline, #p < 0.05 different from the resting leg, ‡p < 0.05 different from Young. Values are presented as means ± SD for 20 participants. For statistical analyses, data for (E) were log‐transformed.