TABLE 1.
Antibody information.
| Target protein | Number | Species | Manufacturer | Dilution | Solution |
|---|---|---|---|---|---|
| p‐mTOR | #2971 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐mTOR | #4517 | Mouse | CST | 1:1000 | TBST 2.5% milk |
| p‐S6K1 | #9234 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐S6K1 | #2708 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| p‐RPS6 | #5364 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐RPS6 | sc‐74 459 | Mouse | SCBT | 1:1000 | TBST 2.5% milk |
| p‐4E‐BP1 | #13442 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐4E‐BP1 | #9452 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| p‐eEF2 | #2331 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐eEF2 | #2332 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| p‐PRAS40 | #2997 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| t‐PRAS40 | sc‐517 639 | Mouse | SCBT | 1:1000 | TBST 2.5% milk |
| Rheb | sc‐130 398 | Mouse | SCBT | 1:500 | TBST 2.5% milk |
| LAT1 | sc‐374 232 | Mouse | SCBT | 1:500 | Fluorescent blocking buffer |
| RagB | #8150 | Rabbit | CST | 1:1000 | TBST 2.5% milk |
| LAMP2 | Sc‐18 822 | Mouse | SCBT | 1:1000 | TBST 2.5% milk |
Abbreviations: 4E‐BP1, initiation factor 4E‐binding protein 1; CST, Cell Signaling Technology; eEF2, eukaryotic elongation factor 2; LAMP2, lysosome‐associated membrane protein 2; LAT1, L‐type amino acid transporter 1; mTOR, mechanistic target of rapamycin; PRAS40, proline‐rich substrate of 40 kDa; Rheb, Ras homologue enriched in brain; RPS6, ribosomal protein S6; S6K1, ribosomal protein S6 kinase 1; SCBT, Santa Cruz Biotechnology; TBST, Tris Buffered Saline with Tween.