Figure 1.

A) Device structure: the device comprises three biologically independent chamber and it is composed of three superimposed layers: a Cell Culture Layer (CCL), a Mechanical Actuation Layer (MAL), and a Glass Coverslip. B) Chamber layout: Each culture chamber is composed of five channels, i.e. two outermost channels for culture medium (channels 1 and 5), two for cell‐laden hydrogels (channels 2 and 4), and one for the injectable therapeutic product (channel 3). C) Working principle. At rest position, cell‐laden hydrogels and the therapeutic product are not mechanically stimulated. Upon pressurization of the MAL, they are subjected to a confined mechanical compression (30%).