Figure 5.

A) Experimental path: hACs embedded in fibrin gel were cultured in the uBeat® MultiCompress Platform for 14 days to achieve mature cartilage constructs. After removing culture medium from therapeutic product‐dedicated channel, SYN321 was injected and co‐cultured with cartilage tissues for 3 days, either in static or dynamic conditions. Culture medium was collected at the end of the culture period for mass spectrometry analysis. B) Cell seeding configurations. In Setup 1 both channels 2 and 4 were injected with cell‐laden hydrogel; in Setup 2 channel 4 was injected with cell‐laden hydrogel and channel 2 was loaded with fibrin only. C) Mass‐Spectrometry Results. Diclofenac concentration in devices cultured with Setup1 and with Setup2, either in static or dynamic conditions. Results are compared with devices loaded with fibrin only in both channels 2 and 4, and with three technical controls, i.e. diclofenac diluted in serum‐free medium and injected in the devices (Chip_SF+D), diclofenac diluted in serum‐free medium without being injected in the devices (SF+D), and diclofenac diluted in DMEM without being injected in the devices (DMEM+D).