Fig. 2.

ATOR inhibits glycolysis and immune escape in glioma cells. A: Effect of ATOR on glucose consumption and lactate production in U87 cells; B: Effect of ATOR on ATP production in U87 cells; C: Western Blot to detect the effect of ATOR on PKM1 and PKM2 in U87 cells; D: Flow cytometry to study the effect of ATOR on T cell-mediated apoptosis in U87 cells; E: ELISA to detect the effect of ATOR on the TNF-α, IFN-γ, and IL-2 secretion in cell cultures; F: Flow cytometry to detect the effect of ATOR on TNF-α and IFN-γ production in CD8⁺ T cells; G: Western Blot to detect the effect of ATOR on PD-L1 expression in U87 cells. Data are expressed as mean ± SD (n = 3). * P < 0.05