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Analysis of DNA extracted from adapted strains of Pp12633 expressing either DEF1 or DEF2. (A) Sequencing coverage of plasmid and defluorinase genes relative to chromosome in preadapted and postadapted DEF1 and DEF2 strains. (B) Agarose gel of plasmids. (C) Schematic of plasmid in DEF2 strain showing region of deletion for the smaller plasmid.
