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. 2023 May 30;4(6):1375–1388. doi: 10.1016/j.fmre.2023.05.008

Table 7.

The mechanism of action of MIF inhibitors.

MIF inhibitors MIF inhibitory activity Mechanism of action Potential Binding sites
Ibudilast Kd = 55.1 µM
Ki = 30.9 µM
MIF tautomerase inhibitor
Anti-inflammation Neuroprotection
Iguratimod IC50 = 6.81 µM MIF tautomerase inhibitor
Anti-inflammation
Immunomodulation
Osteoprotection
Anti-pulmonary fibrosis
ISO-1 IC50 = 7-100 μM MIF tautomerase inhibitor
Anti-inflammation
Neuroprotection
LYS32, ILE64,
ASN97, MET2, PRO1, TYR36,
Z-312 IC50 = 0.55 µM Covalent or slow, tight-binding MIF inhibitor
Anti-inflammation
Z-590 IC50 = 40.54 µM MIF tautomerase inhibitor
Anti-neuroinflammation
Neuroprotection
11 IC50 = 11.95 µM MIF tautomerase inhibitor
Anti-neuroinflammation
4-IPP IC50 = 0.2-0.5 μM Covalent MIF inhibitor
Anti-inflammation
PRO1, TYR36, HIS62, VAL106, PHE113, TYR95, ASN97
PAANIB-1 IC50 = 0.28 μM MIF nuclease inhibitor
Prevention of
neurodegeneration
LEU61
ASN72