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. Author manuscript; available in PMC: 2024 Dec 27.
Published in final edited form as: Adv Healthc Mater. 2024 Sep 19;13(32):e2402199. doi: 10.1002/adhm.202402199

Figure 2. Ventral hSCO differentiation and characterization.

Figure 2.

(A) Schematic illustration of ventral hSCO differentiation protocol. (B, C, D) Immunostaining and flow cytometry analysis for marker expression of hSCO differentiation. (B) and (D) were taken using confocal microscopy. Scale bar = 50 μm. (E) Quantitative RT-PCR for relative mRNA expression of various spinal cord markers after biochemical induction (n=3). (i) Ventral markers; (ii) Interneuron markers; (iii) Dorsal markers. * indicates p≤0.05, **: p≤0.01, ***: p≤0.001.